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alt-r crispr custom guide rnas  (Integrated DNA Technologies)


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    Integrated DNA Technologies alt-r crispr custom guide rnas
    Alt R Crispr Custom Guide Rnas, supplied by Integrated DNA Technologies, used in various techniques. Bioz Stars score: 92/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/alt-r+crispr+custom+guide+rnas/Alt-R+CRISPR+Custom+Guide+RNAs/custom%40alt-r-crispr-custom-guide-rnas%4010%2E1016%2Fj%2Ecelbio%2E2026%2E100537
    Average 92 stars, based on 34 article reviews
    alt-r crispr custom guide rnas - by Bioz Stars, 2026-10
    92/100 stars

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    Related Articles

    Binding Assay:

    Article Title: Quantitative profiling of intrinsic dCas9-DNA recognition reveals key determinants of guide RNA performance
    Article Snippet: PrePrint: as then performed sequentially at 85 °C for 10 mins, 75 °C for 10 mins, 65 °C for 10 mins, and 60 °C for 90 mins. The ribonucleoprotein (RNP) complex was first assembled by incubating 2uM dSpCas9-His (IDT Alt-R™ S.p. dCas9 Protein V3) with 4.8uM sgRNA (IDT Alt-R™ CRISPR Custom Guide RNAs) in the reaction buffer (20 mM Tris-HCl, pH 7.4, 150 mM KCl, 10% glycerol, 5 mM MgCl2 and 1 mM TCEP) for 10 mins

    Incubation:

    Article Title: Quantitative profiling of intrinsic dCas9-DNA recognition reveals key determinants of guide RNA performance
    Article Snippet: PrePrint: as then performed sequentially at 85 °C for 10 mins, 75 °C for 10 mins, 65 °C for 10 mins, and 60 °C for 90 mins. The ribonucleoprotein (RNP) complex was first assembled by incubating 2uM dSpCas9-His (IDT Alt-R™ S.p. dCas9 Protein V3) with 4.8uM sgRNA (IDT Alt-R™ CRISPR Custom Guide RNAs) in the reaction buffer (20 mM Tris-HCl, pH 7.4, 150 mM KCl, 10% glycerol, 5 mM MgCl2 and 1 mM TCEP) for 10 mins

    CRISPR:

    Article Title: Quantitative profiling of intrinsic dCas9-DNA recognition reveals key determinants of guide RNA performance
    Article Snippet: PrePrint: as then performed sequentially at 85 °C for 10 mins, 75 °C for 10 mins, 65 °C for 10 mins, and 60 °C for 90 mins. The ribonucleoprotein (RNP) complex was first assembled by incubating 2uM dSpCas9-His (IDT Alt-R™ S.p. dCas9 Protein V3) with 4.8uM sgRNA (IDT Alt-R™ CRISPR Custom Guide RNAs) in the reaction buffer (20 mM Tris-HCl, pH 7.4, 150 mM KCl, 10% glycerol, 5 mM MgCl2 and 1 mM TCEP) for 10 mins

    Microarray:

    Article Title: Quantitative profiling of intrinsic dCas9-DNA recognition reveals key determinants of guide RNA performance
    Article Snippet: PrePrint: as then performed sequentially at 85 °C for 10 mins, 75 °C for 10 mins, 65 °C for 10 mins, and 60 °C for 90 mins. The ribonucleoprotein (RNP) complex was first assembled by incubating 2uM dSpCas9-His (IDT Alt-R™ S.p. dCas9 Protein V3) with 4.8uM sgRNA (IDT Alt-R™ CRISPR Custom Guide RNAs) in the reaction buffer (20 mM Tris-HCl, pH 7.4, 150 mM KCl, 10% glycerol, 5 mM MgCl2 and 1 mM TCEP) for 10 mins

    Software:

    Article Title: Quantitative profiling of intrinsic dCas9-DNA recognition reveals key determinants of guide RNA performance
    Article Snippet: PrePrint: as then performed sequentially at 85 °C for 10 mins, 75 °C for 10 mins, 65 °C for 10 mins, and 60 °C for 90 mins. The ribonucleoprotein (RNP) complex was first assembled by incubating 2uM dSpCas9-His (IDT Alt-R™ S.p. dCas9 Protein V3) with 4.8uM sgRNA (IDT Alt-R™ CRISPR Custom Guide RNAs) in the reaction buffer (20 mM Tris-HCl, pH 7.4, 150 mM KCl, 10% glycerol, 5 mM MgCl2 and 1 mM TCEP) for 10 mins

    Sequencing:

    Article Title: Quantitative profiling of intrinsic dCas9-DNA recognition reveals key determinants of guide RNA performance
    Article Snippet: PrePrint: as then performed sequentially at 85 °C for 10 mins, 75 °C for 10 mins, 65 °C for 10 mins, and 60 °C for 90 mins. The ribonucleoprotein (RNP) complex was first assembled by incubating 2uM dSpCas9-His (IDT Alt-R™ S.p. dCas9 Protein V3) with 4.8uM sgRNA (IDT Alt-R™ CRISPR Custom Guide RNAs) in the reaction buffer (20 mM Tris-HCl, pH 7.4, 150 mM KCl, 10% glycerol, 5 mM MgCl2 and 1 mM TCEP) for 10 mins

    Injection:

    Article Title: Quantitative profiling of intrinsic dCas9-DNA recognition reveals key determinants of guide RNA performance
    Article Snippet: PrePrint: as then performed sequentially at 85 °C for 10 mins, 75 °C for 10 mins, 65 °C for 10 mins, and 60 °C for 90 mins. The ribonucleoprotein (RNP) complex was first assembled by incubating 2uM dSpCas9-His (IDT Alt-R™ S.p. dCas9 Protein V3) with 4.8uM sgRNA (IDT Alt-R™ CRISPR Custom Guide RNAs) in the reaction buffer (20 mM Tris-HCl, pH 7.4, 150 mM KCl, 10% glycerol, 5 mM MgCl2 and 1 mM TCEP) for 10 mins

    Knock-Out:

    Article Title: Quantitative profiling of intrinsic dCas9-DNA recognition reveals key determinants of guide RNA performance
    Article Snippet: PrePrint: as then performed sequentially at 85 °C for 10 mins, 75 °C for 10 mins, 65 °C for 10 mins, and 60 °C for 90 mins. The ribonucleoprotein (RNP) complex was first assembled by incubating 2uM dSpCas9-His (IDT Alt-R™ S.p. dCas9 Protein V3) with 4.8uM sgRNA (IDT Alt-R™ CRISPR Custom Guide RNAs) in the reaction buffer (20 mM Tris-HCl, pH 7.4, 150 mM KCl, 10% glycerol, 5 mM MgCl2 and 1 mM TCEP) for 10 mins

    Concentration Assay:

    Article Title: Quantitative profiling of intrinsic dCas9-DNA recognition reveals key determinants of guide RNA performance
    Article Snippet: PrePrint: as then performed sequentially at 85 °C for 10 mins, 75 °C for 10 mins, 65 °C for 10 mins, and 60 °C for 90 mins. The ribonucleoprotein (RNP) complex was first assembled by incubating 2uM dSpCas9-His (IDT Alt-R™ S.p. dCas9 Protein V3) with 4.8uM sgRNA (IDT Alt-R™ CRISPR Custom Guide RNAs) in the reaction buffer (20 mM Tris-HCl, pH 7.4, 150 mM KCl, 10% glycerol, 5 mM MgCl2 and 1 mM TCEP) for 10 mins



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    Structural variants by CRISPR/Cas9-mediated nanopore sequencing.

    Journal: Biomedicines

    Article Title: An Integrated Approach Including CRISPR/Cas9-Mediated Nanopore Sequencing, Mate Pair Sequencing, and Cytogenomic Methods to Characterize Complex Structural Rearrangements in Acute Myeloid Leukemia

    doi: 10.3390/biomedicines12030598

    Figure Lengend Snippet: Structural variants by CRISPR/Cas9-mediated nanopore sequencing.

    Article Snippet: Using this method, thirty crRNAs were designed to detect twenty-nine chromosomal abnormalities in chromosomes 3 and 7 ( ). crRNA #28 and #29 were targeted for the same genomic region. crRNAs were designed using Custom Alt-R CRISPR-Cas9 guide RNA ( https://www.idtdna.com/site/order/designtool/index/CRISPR_CUSTOM , 15 February 2020) and Chopchop ( https://chopchop.cbu . uib.no/, 15 February 2020) with CRISPR-Cas9.

    Techniques: CRISPR, Sequencing, Variant Assay, Amplification

    Summarization of various structural variant breakpoints revealed by CRISPR/Cas9-mediated nanopore sequencing, MPseq, and SNP microarray. From left to right, SNP microarray revealed three chromoanagenesis regions: two on chromosome 3q and one on 7q (shown by red circles). Mate pair sequencing data is in a solid red circle, CRISPR/Cas9 nanopore sequencing data is in green circles, and overlapped data of MPseq and CRISPR/cas9 are in brown circles. ( A , B ) Complex SVs in the IGV view. Reads of chromosome 7q11.22 genomic region (69,431,166–69,480,285, A ) and 3q21.3 genomic region (126,248,059–126,263,338, B ). ( C ) Copy number variants by nanopore sequencing and SNP microarray. Reads of chromosome 3q21.1 genomic region (121,889,250–122,611,342) show complex SVs including amplification, gain, and loss detected by nanopore sequencing, which were consistent with SNP microarray findings. Nanopore reads and SNP microarray data were analyzed by the VIA software to generate copy number variants. Chr: chromosome, CNVs: copy number variants, ROIs: regions of interest by CRISPR/cas9 guideRNAs, SVs: structural variants.

    Journal: Biomedicines

    Article Title: An Integrated Approach Including CRISPR/Cas9-Mediated Nanopore Sequencing, Mate Pair Sequencing, and Cytogenomic Methods to Characterize Complex Structural Rearrangements in Acute Myeloid Leukemia

    doi: 10.3390/biomedicines12030598

    Figure Lengend Snippet: Summarization of various structural variant breakpoints revealed by CRISPR/Cas9-mediated nanopore sequencing, MPseq, and SNP microarray. From left to right, SNP microarray revealed three chromoanagenesis regions: two on chromosome 3q and one on 7q (shown by red circles). Mate pair sequencing data is in a solid red circle, CRISPR/Cas9 nanopore sequencing data is in green circles, and overlapped data of MPseq and CRISPR/cas9 are in brown circles. ( A , B ) Complex SVs in the IGV view. Reads of chromosome 7q11.22 genomic region (69,431,166–69,480,285, A ) and 3q21.3 genomic region (126,248,059–126,263,338, B ). ( C ) Copy number variants by nanopore sequencing and SNP microarray. Reads of chromosome 3q21.1 genomic region (121,889,250–122,611,342) show complex SVs including amplification, gain, and loss detected by nanopore sequencing, which were consistent with SNP microarray findings. Nanopore reads and SNP microarray data were analyzed by the VIA software to generate copy number variants. Chr: chromosome, CNVs: copy number variants, ROIs: regions of interest by CRISPR/cas9 guideRNAs, SVs: structural variants.

    Article Snippet: Using this method, thirty crRNAs were designed to detect twenty-nine chromosomal abnormalities in chromosomes 3 and 7 ( ). crRNA #28 and #29 were targeted for the same genomic region. crRNAs were designed using Custom Alt-R CRISPR-Cas9 guide RNA ( https://www.idtdna.com/site/order/designtool/index/CRISPR_CUSTOM , 15 February 2020) and Chopchop ( https://chopchop.cbu . uib.no/, 15 February 2020) with CRISPR-Cas9.

    Techniques: Variant Assay, CRISPR, Nanopore Sequencing, Microarray, Sequencing, Amplification, Software

    Comparison of data of SNP microarray, MPseq, and CRISPR/Cas9-mediated nanopore sequencing.

    Journal: Biomedicines

    Article Title: An Integrated Approach Including CRISPR/Cas9-Mediated Nanopore Sequencing, Mate Pair Sequencing, and Cytogenomic Methods to Characterize Complex Structural Rearrangements in Acute Myeloid Leukemia

    doi: 10.3390/biomedicines12030598

    Figure Lengend Snippet: Comparison of data of SNP microarray, MPseq, and CRISPR/Cas9-mediated nanopore sequencing.

    Article Snippet: Using this method, thirty crRNAs were designed to detect twenty-nine chromosomal abnormalities in chromosomes 3 and 7 ( ). crRNA #28 and #29 were targeted for the same genomic region. crRNAs were designed using Custom Alt-R CRISPR-Cas9 guide RNA ( https://www.idtdna.com/site/order/designtool/index/CRISPR_CUSTOM , 15 February 2020) and Chopchop ( https://chopchop.cbu . uib.no/, 15 February 2020) with CRISPR-Cas9.

    Techniques: Comparison, Microarray, CRISPR, Sequencing, Variant Assay, Nanopore Sequencing, Amplification